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KMID : 1007520190280061747
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Food Science and Biotechnology
2019 Volume.28 No. 6 p.1747 ~ p.1757
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Vital parameters for high gamma-aminobutyric acid (GABA) production by an industrial soy sauce koji Aspergillus oryzae NSK in submerged-liquid fermentation
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Wan-Mohtar Wan Abd Al Qadr Imad
Kadir Safuan Ab
Halim-Lim Sarina Abdul
Ilham Zul
Hajar-Azhari Siti
Saari Nazamid
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Abstract
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In submerged-liquid fermentation, seven key parameters were assessed using one-factor-at-a-time to obtain the highest GABA yield using an industrial soy sauce koji Aspergillus oryzae strain NSK (AOSNSK). AOSNSK generated maximum GABA at 30 °C (194 mg/L) and initial pH 5 (231 mg/L), thus was able to utilize sucrose (327 mg/L of GABA) for carbon source. Sucrose at 100 g/L, improved GABA production at 646 mg/L. Single nitrogen sources failed to improve GABA production, however a combination of yeast extract (YE) and glutamic acid (GA) improved GABA at 646.78 mg/L. Carbon-to-nitrogen ratio (C8:N3) produced the highest cell (24.01 g/L) and GABA at a minimal time of 216 h. The key parameters of 30 °C, initial pH 5, 100 g/L of sucrose, combination YE and GA, and C8:N3 generated the highest GABA (3278.31 mg/L) in a koji fermentation. AOSNSK promisingly showed for the development of a new GABA-rich soy sauce.
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KEYWORD
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Aspergillus oryzae, Gamma-aminobutyric acid (GABA), Soy sauce koji, Glutamic acid, GABA-enriched foods
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